Cell Function Reagents and Kits
Invitrogen™ 5-(and-6)-Carboxy SNARF™-1
The cell-impermeant pH indicator has pKa of approximately 7.5, thus is useful for measuring pH changes between pH 7 and pH 8
Invitrogen™ Escherichia coli (K-12 strain) BioParticles™, Texas Red™ conjugate
The fluorescence of the dye conjugates is uniformly intense over the pH range from 3 to 10.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ Fura-2 AM Dye
Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.
Invitrogen™ eBioscience™ JC-1 Mitochondrial Membrane Potential Dye
Used to determine mitochondrial membrane potential in flow cytometry and fluorescent microscopy. Dye selectively enters mitochondria where it reversibly changes color as membrane potentials increase. Property due to the reversible formation of JC-1 aggregates upon membrane polarization.
Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 488 conjugate
Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ Lipopolysaccharides From Escherichia coli Serotype 055:B5, Alexa Fluor™ 488 Conjugate
This bright, green-fluorescent conjugate can be used to follow lipopolysaccharide binding, transport and cell-internalization processes
Invitrogen™ Low Density Lipoprotein from Human Plasma, BODIPY™ FL complex (BODIPY™ FL LDL)
Nonacetylated conjugate
Invitrogen™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably, and quantitatively distinguish live and dead yeast in minutes by flow cytometry.
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)
A two-color fluorescent cell viability assay that simultaneously identifies live and dead cells. Live cells are labeled with calcein dye and emit a green fluorescence, while dead cells are labeled with SYTOX Deep Red dye and emit a red fluorescence.
Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated, DiI complex (DiI AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells
